Features
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Animal-derived component-free
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Protein-free/Peptide ingredients-free
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Chemically Defined
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DMF Filing in Progress
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Universality: Support the culture of various CHO cell lines (CHO-K1, CHO_ZN, Horizon, CHO-S, CHO DG44, etc.) in fed-batch, intensified fed-batch and perfusion modes
Advantages
Equivalent or superior performance to global brand products with better price and lead time
4 advanced CMPM (Cone Mixing Pin Milling) powder media production lines to meet different customer demand with high inter-batch consistency (CPK*>1.33)
Safe and compliant: chemically defined formulation minimizes the potential of contamination from animal-derived components and meets relevant compliance and regulatory requirements
EU certified ISO13485:2016 QMS and MDSAP (FDA), all data are traceable
Full documentation support available for IND application
Strictly selected the "2 domestic+1 imported" raw material supplier model to to ensure a stable and reliable supply chain
*CPK is a standard index to state the capability of one process. CPK=1.33: the process is capable and meets specification limits. The higher the CPK,the better.
Order Information
Basal Medium
Fed-Batch Cultures
Application | Product Name | Cat. No. | Size | Form | Instructions (PDF) | Inquiries /Sample Applications |
CHO-K1, CHO-ZN, HORIZON, CHO-S, and CHO DG44, etc. | Eden B600S Basal Medium | EXP0109901 | 10 L | Powder | Download | |
EXP0109902 | 200 L | Powder | ||||
Eden B501S Basal Medium | EXP0106001 | 10 L | Powder | Download | ||
EXP0106002 | 200 L | Powder | ||||
Eden B401S Basal Medium | EXP0105901 | 10 L | Powder | Download | ||
EXP0105902 | 100 L | Powder | ||||
EXP0105903 | 200 L | Powder | ||||
Eden B300S Basal Medium | EXP0107901 | 100 L | Powder | Download | ||
EXP0107902 | 200 L | Powder | ||||
EXP0107903 | 10 L | Powder |
Application | Product Name | Cat. No. | Size | Form | Product Instruction (pdf) | |
CHO-K1, CHO-ZN, HORIZON, CHO-S, and CHO DG44, etc. | Eden F601aS Feed Medium | EXP0112101 | 20 L | Powder | Download | |
EXP0112102 | 10 L | Powder | ||||
EXP0112103 | 1 L | Powder | ||||
Eden F600aS Feed Medium | EXP0108901 | 20 L | Powder | Download | ||
EXP0108902 | 10 L | Powder | ||||
EXP0108903 | 1 L | Powder | ||||
Eden F500aS Feed Medium | EXP0105001 | 1 L | Powder | Download | ||
EXP0105002 | 20 L | Powder | ||||
Eden F400aS Feed Medium | EXP0104801 | 1 L | Powder | Download | ||
EXP0104802 | 20 L | Powder | ||||
Eden F600bS Feed Medium | EXP0108801 | 10 L | Powder | Download | ||
EXP0108802 | 1 L | Powder | ||||
Eden F200bS Feed Medium | EXP0104601 | 1 L | Powder | Download | ||
EXP0104602 | 10 L | Powder | ||||
EXP0104603 | 20 L | Powder | ||||
EXP0104604 | 50 L | Powder |
Performance
Antibodies
In fed-batch process, CHO cells cultured in Eden CD CHO media demonstrate higher viable cell density (VCD) and protein production than competitors. On average, the antibody titers of various CHO cell lines cultured in Eden CD CHO media ranged from 2 to 11 g/L.
In the pulse perfusion process, CHO cells cultured in Eden CD CHO media demonstrated superior performance than competitive global brands. When VVD=1.0, volumetric productivity (Vp) can reach up to 2.2 g/L/day, and the cumulative product expression in 14 days can reach 19 g/L, 55% higher than global brand B. When VVD=2.0, Vp can reach up to 3.3g/L/day, and the cumulative product expression in 14 days can reach 25 g/L.
Documents
CHO Cell Medium
FAQ
Q1:Does the medium require pH adjustment during preparation?
Q2:Is BioEngine's CHO cell culture medium the same as AGT medium?
Q3:What is the lead time for product delivery?
Q4:What is in DMEM media?
Q5: What are the differences between transient and stable protein expression?
Transient protein expression involves the introduction of foreign DNA into cells, which leads to the transient production of the protein of interest. This can be achieved through the use of viral vectors or by introducing plasmids encoding the protein into the cells. Transient protein expression typically results in high levels of protein expression over a short period of time, typically a few days. This makes it a useful technique for rapid screening of proteins or for the production of small quantities of protein for research purposes.
Stable protein expression, on the other hand, involves the integration of foreign DNA into the host cell genome, resulting in the long-term production of the protein of interest. This can be achieved through the use of viral vectors or by selecting for cells that have incorporated the foreign DNA. Stable protein expression typically results in lower levels of protein expression than transient expression but can be maintained over long periods of time. This makes it a useful technique for the production of larger quantities of protein for research or commercial purposes.
Another difference between transient and stable protein expression is the level of control over protein expression. In transient expression, the level of protein expression is typically high and difficult to control, while in stable expression, the level of protein expression can be controlled by selecting for cells with the desired level of expression or by using inducible expression systems.
Overall, the choice between transient and stable protein expression will depend on the specific requirements of the experiment or application, including the desired quantity and quality of the protein, the duration of expression, and the level of control required over protein expression.
Q6: What are the differences between 293 cells and CHO cells in protein production?
1. Expression system: 293 cells are human embryonic kidney cells, while CHO cells are Chinese hamster ovary cells. As a result, 293 cells are a human expression system, while CHO cells are a non-human expression system.
2. Transfection efficiency: 293 cells have a higher transfection efficiency than CHO cells, meaning that they are more likely to take up and express exogenous DNA. This can result in higher levels of protein expression.
3. Glycosylation patterns: CHO cells are known for their ability to perform complex glycosylation, which is important for the biological activity and stability of many therapeutic proteins. 293 cells, on the other hand, tend to produce simpler glycosylation patterns.
4. Protein quality: Due to their ability to perform complex glycosylation, CHO cells often produce higher quality and more stable proteins than 293 cells. However, the higher transfection efficiency of 293 cells can compensate for this in some cases.
Overall, both 293 cells and CHO cells have their advantages and disadvantages for protein production, and the choice of cell line will depend on the specific needs of the project.
Q7: What are the advantages of hybridoma cells in antibody production?
They have natural affinity maturation, which means they have undergone genetic modifications in the host animal to optimize their antibody binding.
They have low level of immunogenicity, which means they are less likely to trigger an immune response in the recipient.
They have constant domain activity, which means they can interact with other immune cells and molecules through their constant regions.
They offer limitless production of homogenous antibodies that are highly sensitive and specific.
They are cost-effective compared to other methods of producing mAbs.
They are useful for diagnostic, imaging, and therapeutic purposes in various fields of research and medicine.
Q8: What is a hybridoma cell?
Q9: How to prevent mycoplasma contamination during cell culture?
1. Practice good aseptic technique: Use proper sterile technique when handling cells and cell culture media. Use sterile equipment, including pipettes, tips, and culture dishes. Keep the work area clean and free from potential sources of contamination.
2. Test incoming cell lines for mycoplasma: Before starting a new cell line, test it for mycoplasma using a commercially available mycoplasma detection kit or other methods.
3. Quarantine new cell lines: Isolate new cell lines in a separate area of the lab until they have been tested and confirmed to be free of mycoplasma contamination.
4. Regularly monitor cell cultures for mycoplasma: Routinely test cell cultures for mycoplasma contamination using a commercial kit or other methods. It is recommended to test every 4-6 weeks for mycoplasma contamination.
5. Use antibiotics: Add antibiotics to the cell culture medium to prevent mycoplasma contamination. Common antibiotics used for this purpose include penicillin, streptomycin, and gentamicin.
6. Store and handle cell cultures properly: Store cell cultures at the appropriate temperature and humidity conditions. Avoid overcrowding of cultures and keep incubators clean.
7. Limit exposure of cell cultures to outside sources: Minimize the exposure of cell cultures to sources of contamination such as skin, hair, and dust.
By following these measures, it is possible to reduce the risk of mycoplasma contamination in cell culture and maintain healthy cell cultures."
Q10:What are the advantages of BHK cells?
1. High transfection efficiency: BHK cells are highly transfectable and can be easily genetically manipulated to express various proteins or molecules of interest.
2. Adaptable to serum-free culture: BHK cells can be adapted to grow in serum-free or chemically defined media, which is essential for bioprocessing applications.
3. High protein production: BHK cells have been widely used for the production of recombinant proteins, such as monoclonal antibodies, due to their ability to produce high yields of secreted proteins.
4. Easy to maintain and scale-up: BHK cells are easy to maintain in culture and can be scaled up for large-scale bioprocessing applications.
5. Broad applications: BHK cells have been used for various applications, including vaccine production, gene therapy, and other biopharmaceutical production."
Q11: What is the McCoy 5A medium without phenol red?
Q12: What is the MDCK cell line culture?
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